File name: Hplc Troubleshooting Pdf
Rating: 4.4 / 5 (4055 votes)
Downloads: 14306
========================
========================
StepCheck wavelength calibration A Few Clicks to Find the Probable Cause. Pump Injector/Autosampler Column Detector Data System/Integrator All of these components can have problems and require troubleshooting. Rectifying • Check pressure with/without columnmany pressure problems are due to blockages in the system or at the guard If Column pressure is high: Wash column – Eliminate HPLC. Because the issue is related to peaks, select “Peaks” (Figure 2b). A. Injection Solvent % Acetonitrile B. Injection Solvent Mobile Phase. Deuterium Lamp (hours)Deuterium Lamp (hours): *. Isolation of the exact cause of the problem. Tip: Injecting in a solvent stronger than the mobile phase can cause peak shape problems such as peak splitting or broadening Trick: Keep Organic Concentration in Sample Solvent < Mobile Phase HPLC Troubleshooting Uwe D. Neue, Waters CorporationColumn Life-TimeVariable Retention TimesDrifting Retention TimesColumn-to-Column and Batch-to-Batch ReproducibilitySample Preparation ProblemsSources of Peak TailingNormal-Phase ChromatographySystem Volume, Dead Volume, Dwell VolumeTransfer of Gradient Replacing the DAD/MWD Lamps. Locate the table for the type of problem you have, find the possible cause and use the short File Size: KB A Few Clicks to Find the Probable Cause. Using the problem of negative peaks described above, the HPLC Troubleshooting Guide finds a solution as follows: From the app’s Any troubleshooting strategy involves five steps: Identification of the problem. Sli ategories of Column Problems. If you have a problem you need to solve now, use these Troubleshooting Tables. StepRemove the lamp by unscrewing both screws and unplugging it. Awareness of the cause(s) of the problem. ContentsGENERAL (U)HPLC TROUBLE SHOOTING APPROACHPROBLEMS IN (U)HPLC AND THEIR CAUSE/CORRECTIONBack pressure CaffeineSalicylamide. A. Pressure B. Peak shape C. Retention. (U)HPLC. StepInstall the lamp into the housing (auto-aligning) and tighten screws. Slide 7 SlideHPLC Components. Choose “Negative peaks” (Figure 2c) HPLC Column Troubleshooting What Every HPLC User Should Know. Using the problem of negative peaks described above, the HPLC Troubleshooting Guide finds a solution as follows: From the app’s home screen, select “Start Troubleshooting” (Figure 2a).